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Quantification of Hypopigmentation Activity In Vitro
Published on: March 6, 2019
Melanogenesis stimulation in murine B16 melanoma cells by Piper nigrum leaf extract and its lignan constituents
Hideaki Matsuda1, Yoshiko Kawaguchi, Miho Yamazaki
1School of Pharmaceutical Sciences, Kinki University, Higashiosaka, Osaka, Japan. matsuda@phar.kindai.ac.jp
Abstract:
A methanolic extract from the leaves of Piper nigrum L. showed a significant stimulatory effect on melanogenesis in cultured murine B16 melanoma cells. Activity-guided fractionation of the methanolic extract led to the isolation of two known lignans, (-)-cubebin (1) and (-)-3,4-dimethoxy-3,4-desmethylenedioxycubebin (2), together with a new lignan, (-)-3-desmethoxycubebinin (3). Among these lignans, 1 and 2 showed a significant stimulatory activity of melanogenesis without any significant effects on cell proliferation.
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