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Related Experiment Videos

von Willebrand factor expression in osteosarcoma metastasis.

Kolja Eppert1, Jay S Wunder, Vicky Aneliunas

  • 1Fred A Litwin Centre for Cancer Genetics, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada.

Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc
|October 7, 2004
PubMed
Summary

Von Willebrand factor (vWF) is increasingly expressed in osteosarcoma tumors, particularly in metastases. This suggests vWF may play a role in the progression of this bone cancer.

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Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Osteosarcoma progression involves multiple genes, with some yet unidentified.
  • Von Willebrand factor (vWF) is crucial for platelet aggregation and metastasis.
  • vWF was traditionally thought to be expressed only in endothelial cells and megakaryocytes.

Purpose of the Study:

  • To investigate the role of von Willebrand factor (vWF) in osteosarcoma progression and metastasis.
  • To examine vWF gene expression in primary and metastatic osteosarcoma tumors.
  • To determine if vWF is expressed by osteosarcoma cells themselves.

Main Methods:

  • Gene expression profiling of 22 osteosarcoma tumors.
  • Analysis of paired primary and metastatic samples.

Related Experiment Videos

  • Immunohistochemistry to detect vWF protein in tumor cells and assess vascular density.

    • Main Results:

    • vWF mRNA expression was significantly increased in metastatic osteosarcoma compared to primary tumors (P=0.005).
    • vWF protein was detected in osteosarcoma cells in 13 of 29 tumor specimens and in an osteosarcoma cell line.
    • Tumor cell vWF staining correlated positively with overall vWF expression (P=0.006).
    • No correlation was found between vascular density and vWF expression or clinical outcome.

    Conclusions:

    • vWF expression is deregulated in osteosarcoma.
    • Osteosarcoma cells can express vWF, suggesting an autocrine or paracrine role.
    • Increased vWF expression may contribute to osteosarcoma metastasis.