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Updated: Jul 20, 2026

Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes
Published on: November 1, 2012
Stacking and hydrogen bonding: DNA cooperativity at melting
Vladimir F Morozov1, Artem V Badasyan, Arsen V Grigoryan
1Department of Molecular Physics, Yerevan State University, A. Manougian Str.1, 375025, Yerevan, Armenia. morozov@ysu.am
Abstract:
By taking into account base-base stacking interactions we improve the Generalized Model of Polypeptide Chain (GMPC). Based on a one-dimensional Potts-like model with many-particle interactions, the GMPC describes the helix-coil transition in both polypeptides and polynucleotides. In the framework of the GMPC we show that correctly introduced nearest-neighbor stacking interactions against the background of hydrogen bonding lead to increased stability (melting temperature) and, unexpectedly, to decreased cooperativity (maximal correlation length). The increase in stability is explained as due to an additional stabilizing interaction (stacking) and the surprising decrease in cooperativity is seen as a result of mixing of contributions of hydrogen bonding and stacking.
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