Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

New hydrolyzable pH-responsive cationic polymers for gene delivery: a preliminary study.

Laurent Veron1, Arnaud Ganée, Marie-Thérèse Charreyre

  • 1Unité Mixte CNRS-bioMérieux, UMR 2142, ENS-Lyon, 46 allée d'Italie, 69364 Lyon Cedex 07, France.

Macromolecular Bioscience
|October 7, 2004
PubMed
Summary

This study introduces a new methacrylate monomer, IPEMA, with a hydrolyzable imidazole side chain. Copolymers exhibit proton sponge behavior and DNA complexing properties, showing potential for gene delivery applications.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Initiating transformative medical practices to address environmental challenges - proceedings of "Santé en 2050", the French national conference on healthcare adaptation.

Reviews on environmental health·2026
Same author

Chain-End Grafting of RAFT Polyelectrolyte Macroligands to Stabilize Near-Infrared-Emitting Gold Nanoclusters.

Macromolecular rapid communications·2026
Same author

Data from long-term experiments in temperate croplands to evaluate soil organic carbon models.

Scientific data·2026
Same author

Planetary health diet with einkorn as a potential preventive strategy to improve interdental microbiota, oral health and quality of life: a pilot clinical trial.

Journal of oral microbiology·2026
Same author

Behavioral Profiles and Sociodemographic Predictors of Planetary Health Diet Engagement Among Health Care Professionals to Inform Public Health Promotion: Cross-Sectional Study.

JMIR public health and surveillance·2025
Same author

Participatory assessment of minor crops: A situated study on hulled wheats.

Quantitative plant biology·2025

Area of Science:

  • Polymer Chemistry
  • Organic Synthesis
  • Materials Science

Background:

  • Methacrylate derivatives are versatile building blocks in polymer science.
  • Imidazole-containing polymers offer unique properties like proton buffering and potential biological interactions.
  • Developing novel monomers with tailored functionalities is crucial for advanced material applications.

Purpose of the Study:

  • To synthesize and characterize a novel methacrylate monomer, 2-methylacrylic acid 2-(3-imidazol-1-yl-propionyloxy)ethyl ester (IPEMA).
  • To investigate the polymerization behavior (homo- and copolymerization) of IPEMA.
  • To evaluate the functional properties of the resulting imidazole-containing copolymers, including proton sponge capacity and DNA complexation.

Main Methods:

  • Synthesis and characterization of the IPEMA monomer.

Related Experiment Videos

  • Free radical homo- and copolymerization studies in N,N-dimethylformamide.
  • Determination of reactivity ratios for IPEMA and N,N-dimethylaminoethyl methacrylate (DMAEMA) copolymerization.
  • Molecular weight analysis and refractive index increment measurements.
  • Potentiometric titrations and preliminary hydrolysis studies.
  • Agarose gel electrophoresis for DNA complexation assessment.

    • Main Results:

    • Successful synthesis and characterization of IPEMA monomer.
    • Determination of kinetic parameters (kp/kt(1/2)) for IPEMA homopolymerization.
    • Quantification of reactivity ratios for IPEMA/DMAEMA copolymerization (r(DMAEMA) = 1.13 +/- 0.09, r(IPEMA) = 0.82 +/- 0.09).
    • Demonstrated proton sponge ability and slow hydrolysis of imidazole units from copolymers.
    • Evidence of DNA complexing properties of the imidazole-based copolymers.

    Conclusions:

    • IPEMA is a novel methacrylate monomer with a functional imidazole side chain.
    • The synthesized copolymers possess 'proton sponge' characteristics and can complex with DNA.
    • These imidazole-containing copolymers show promise for applications requiring pH responsiveness and nucleic acid delivery.