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Updated: Apr 17, 2026

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

Published on: January 9, 2017

Single-particle sizing from light scattering by spectral decomposition.

Konstantin A Semyanov¹, Peter A Tarasov, Alexey E Zharinov

¹Institute of Chemical Kinetics and Combustion, Siberian Branch of the Russian Academy of Sciences, Institutskaya 3, Novosibirsk 630090 Russia.

|October 8, 2004

Summary

This study introduces a new method using Fourier transforms to accurately determine spherical particle size from light scattering patterns. The technique precisely measures particle diameters, offering a valuable tool for scientific analysis.

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Area of Science:

Optical physics
Particle characterization
Metrology

Background:

Accurate particle size determination is crucial in various scientific fields.
Traditional methods may have limitations in precision or applicability.

Purpose of the Study:

To develop and validate a novel method for sizing individual spherical particles using angular light-scattering patterns.
To establish a reliable relationship between particle size and spectral peak location.

Main Methods:

Application of Fourier transform to angular light-scattering data.
Regression analysis of simulated patterns to derive a size-dependent equation.
Validation using polystyrene microspheres and erythrocytes with a scanning flow cytometer.

Main Results:

A strong correlation was found between particle size and the peak position in the amplitude spectrum.
The method accurately characterizes particles with size parameters from 8 to 180 (1.2–27.2 µm).
Achieved precision of 60 nm for particle sizing within a refractive index range of 1.35–1.70.

Conclusions:

The developed Fourier transform method offers a precise and accurate approach for spherical particle sizing.
The technique demonstrates broad applicability across various particle types and sizes.
This method provides a valuable alternative for particle characterization in scientific research.