Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA microarray probe preparation by gel isolation nested PCR.

Hong-min Wang1, Wen-li Ma, Hai Huang

  • 1Institute of Molecular Biology, First Uniform Medical University, Guangzhou 510515, P.R. China.

Journal of Biochemistry and Molecular Biology
|October 8, 2004
PubMed
Summary

A new GIN-PCR method simplifies DNA microarray probe preparation using gel-isolated PCR products and a wooden toothpick. This rapid, cost-effective technique enables efficient DNA microarray fabrication and hybridization.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effect of Zn Addition on the Cd-Containing Anaerobic Fermentation Process: Biodegradation and Microbial Communities.

International journal of environmental research and public health·2019
Same author

Glass stabilized ultra-stable dual-emitting Mn-doped cesium lead halide perovskite quantum dots for cryogenic temperature sensing.

Nanoscale·2019
Same author

Simultaneous Tailoring of Dual-Phase Fluoride Precipitation and Dopant Distribution in Glass to Control Upconverting Luminescence.

ACS applied materials & interfaces·2019
Same author

<i>Nkx2-5</i> defines a subpopulation of pacemaker cells and is essential for the physiological function of the sinoatrial node in mice.

Development (Cambridge, England)·2019
Same author

Process Analysis of Anaerobic Fermentation Exposure to Metal Mixtures.

International journal of environmental research and public health·2019
Same author

Surface-to-Bulk Redox Coupling through Thermally Driven Li Redistribution in Li- and Mn-Rich Layered Cathode Materials.

Journal of the American Chemical Society·2019

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Genomics

Background:

  • DNA microarrays are crucial tools in molecular biology and genomics.
  • Efficient and large-scale preparation of DNA microarray probes is essential for advancing research.
  • Current methods for probe preparation can be labor-intensive and time-consuming.

Purpose of the Study:

  • To develop a simplified, rapid, and cost-effective method for large-scale DNA microarray probe preparation.
  • To introduce a novel nested PCR technique mediated by gel isolation of PCR products.
  • To evaluate the efficiency and effectiveness of the developed probe preparation method.

Main Methods:

  • Construction of a lambda phage genomic DNA-fragments library for probe collection.
  • Comparison of four DNA band recovery methods from initial PCR products.

Related Experiment Videos

  • Development and application of a novel GIN-PCR (Gel Isolation-mediated Nested PCR) technique.
  • Fabrication of DNA microarrays using probes prepared by the GIN-PCR method.
  • Evaluation of probe preparation using a wooden toothpick for DNA band transfer.

    • Main Results:

    • The wooden toothpick method proved superior for transferring DNA bands as templates for nested PCR.
    • Successful collection of DNA microarray probes using the GIN-PCR technique.
    • Fabrication of DNA microarrays with the prepared probes.
    • Hybridization experiments confirmed the rapid, efficient, and effective nature of the GIN-PCR method.
    • The developed method is cost-effective and less labor-intensive.

    Conclusions:

    • The GIN-PCR technique offers a significant improvement for DNA microarray probe preparation.
    • The use of a wooden toothpick for DNA band transfer enhances efficiency and reliability.
    • This simplified method facilitates rapid, large-scale production of DNA microarray probes.
    • The developed approach is highly effective for DNA microarray fabrication and subsequent analyses.