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Transendothelial migration of human basophils
Motoyasu Iikura1, Motohiro Ebisawa, Masao Yamaguchi
1Department of Allergy and Rheumatology, University of Tokyo Graduate School of Medicine, Hongo, Bunkyo-ku, Japan. iikura-tky@umin.ac.jp
Journal of Immunology (Baltimore, Md. : 1950)
|October 8, 2004
Summary
Basophil transendothelial migration (TEM) is crucial for allergic reactions. This study shows IL-1beta and chemokines like eotaxin enhance basophil TEM, primarily involving beta2 integrins, similar to eosinophils.
Area of Science:
- Immunology
- Cell Biology
- Allergy Research
Background:
- Basophils are key effector cells in allergic reactions, migrating from blood to inflammatory sites.
- Transendothelial migration (TEM) is a critical step for basophil extravasation.
- Understanding basophil TEM mechanisms is essential for developing targeted allergy therapies.
Purpose of the Study:
- To investigate the mechanisms of basophil transendothelial migration (TEM) using human umbilical vein endothelial cells (HUVEC).
- To identify specific cytokines, chemokines, and cell adhesion molecules involved in basophil TEM.
- To compare the regulatory profile of basophil TEM with that of eosinophils.
Main Methods:
- Studied basophil TEM across activated HUVEC monolayers.
- Utilized various stimuli including IL-1beta, eotaxin/CCL11, MCP-1/CCL2, and stromal cell-derived factor-1/CXCL12.
- Assessed the role of integrins (CD18, CD29) and adhesion molecules (ICAM-1, VCAM-1) using blocking antibodies.
Main Results:
- IL-1beta significantly enhanced basophil TEM, further potentiated by eotaxin/CCL11 and MCP-1/CCL2.
- Stromal cell-derived factor-1/CXCL12 induced TEM in cultured basophils but not freshly isolated ones.
- IL-3 increased basophil TEM by enhancing chemokinesis; beta2 integrins played a primary role, with beta1 integrins also involved in cytokine/chemokine-stimulated TEM.
Conclusions:
- Basophil TEM is regulated by multiple cytokines and chemokines, with beta2 integrins being crucial.
- The regulatory profile of basophil TEM closely resembles that of eosinophils.
- These findings suggest similar in vivo migration kinetics for basophils and eosinophils in inflammatory responses.