Related Experiment Video
Updated: Aug 21, 2026
Antigen-Capture Enzyme-Linked Immunosorbent Assay for Specific Detection of Mycoplasma pneumoniae
Published on: February 24, 2023
Serological study of Mycoplasma pneumoniae infections
Somporn Srifuengfung1, Wanida Techachaiwiwat, Chertsak Dhiraputra
1Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Abstract:
Mycoplasma pneumoniae antibody was determined in 811 sera of different patients admitted to Siriraj Hospital with respiratory tract infection from July 1, 2000 to August 31, 2003 by agglutination with gelatin particle agglutination test kit (SERODIA-MYCO II, Fujirebio Inc. Japan) in microtiter plates. Three hundred and three sera were positive (37.36%). The five most positive titer were found in patients 5-9 yr (40.26%), followed by patients 1-4 yr (24.75%), 10-14 yr (19.80%), 30-39 yr (5.28%) and 20-29 yr (3.96%). The positive titers ranged from 40 to > 20,480. Female:male ratio in positive patients was approximately the same (1.19:1). High titers (> or = 320) were found in 146 out of 303 patients (48.18%). The infection was mostly found in children aged 5-9 yr. Detection of antibody to M. pneumoniae infection showed that 37.36% of patients who were suspected of having atypical bacterial pneumonia were positive.
Insights
A study found that 37.36% of patients with respiratory infections had Mycoplasma pneumoniae antibodies. Children aged 5-9 years showed the highest positive rates for this common bacterial pneumonia cause.
Area of Science:
- Infectious Diseases
- Microbiology
- Immunology
Background:
- Mycoplasma pneumoniae is a significant cause of community-acquired pneumonia, particularly in children.
- Accurate diagnosis is crucial for effective treatment and public health management of M. pneumoniae infections.
Purpose of the Study:
- To determine the prevalence of Mycoplasma pneumoniae antibodies in patients with respiratory tract infections.
- To identify the age groups most affected by M. pneumoniae infections.
Main Methods:
- A gelatin particle agglutination test kit (SERODIA-MYCO II) was used to detect M. pneumoniae antibodies.
- The study analyzed 811 serum samples from patients admitted to Siriraj Hospital between July 2000 and August 2003.
Main Results:
- Antibodies to M. pneumoniae were detected in 37.36% (303/811) of the patients.
- The highest prevalence was observed in children aged 5-9 years (40.26% of positive cases), followed by those aged 1-4 years (24.75%).
- High antibody titers (≥320) were found in 48.18% of positive patients.
Conclusions:
- Mycoplasma pneumoniae is a common pathogen in patients presenting with respiratory tract infections, especially in young children.
- The findings highlight the importance of considering M. pneumoniae in the differential diagnosis of atypical bacterial pneumonia.
