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Nucleotide sequence and organization of the multiresistance plasmid pSCFS1 from Staphylococcus sciuri
Corinna Kehrenberg1, Kayode K Ojo, Stefan Schwarz
1Institut für Tierzucht, Bundesforschungsanstalt für Landwirtschaft (FAL), Höltystrasse 10, 31535 Neustadt-Mariensee, Germany.
The Journal of Antimicrobial Chemotherapy
|October 9, 2004
Summary
The Staphylococcus sciuri plasmid pSCFS1 harbors multiple resistance genes, including novel ones. A newly identified ABC transporter on this plasmid contributes to lincosamide antimicrobial resistance.
Area of Science:
- Microbiology
- Molecular Biology
- Genetics
Background:
- The emergence of antimicrobial resistance is a significant global health threat.
- Plasmids play a crucial role in the dissemination of resistance genes among bacteria.
- Understanding the genetic basis of plasmid-mediated resistance is essential for developing new control strategies.
Purpose of the Study:
- To fully sequence and analyze the multiresistance plasmid pSCFS1 from Staphylococcus sciuri.
- To elucidate the gene organization of pSCFS1.
- To investigate the role of a novel ABC transporter in antimicrobial resistance.
Main Methods:
- Complete sequencing of plasmid pSCFS1.
- Transformation of pSCFS1 into Staphylococcus aureus RN4220.
- Cloning of restriction fragments into Escherichia coli plasmid vectors for sequencing.
- MIC determination of S. aureus transformants harboring specific pSCFS1 fragments.
Main Results:
- The 17,108 bp sequence of pSCFS1 revealed 14 open reading frames (ORFs).
- Genes conferring resistance to chloramphenicol/florfenicol (cfr) and macrolide-lincosamide-streptogramin B (erm(33)) were identified, along with spectinomycin resistance and transposase genes.
- A novel ABC transporter was discovered and demonstrated to confer low-level lincosamide resistance.
Conclusions:
- Plasmid pSCFS1 contains a mosaic of genetic elements found in both Gram-positive and Gram-negative bacteria.
- The plasmid likely resulted from inter-plasmid recombination events.
- Truncation of a Tn554-like transposon may have contributed to the pSCFS1 structure.