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Related Experiment Videos

Multilocus variable-number tandem-repeat polymorphism among Brazilian Enterococcus faecalis strains.

Ricardo Titze-de-Almeida1, Rob J L Willems, Janetta Top

  • 1Molecular Microbiology and Biotechnology, University of Brasília, Brasilia-DF, Brazil. titze@unb.br

Journal of Clinical Microbiology
|October 9, 2004
PubMed
Summary
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Multilocus variable-number tandem-repeat analysis (MLVA) provides a reliable method for tracking Enterococcus faecalis strains. This molecular typing technique is a highly concordant and excellent alternative to pulsed-field gel electrophoresis (PFGE).

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Enterococcus faecalis is an important opportunistic pathogen.
  • Accurate bacterial strain typing is crucial for infection control and epidemiology.
  • Pulsed-field gel electrophoresis (PFGE) is a common but labor-intensive typing method.

Purpose of the Study:

  • To develop and evaluate a Multilocus variable-number tandem-repeat analysis (MLVA) scheme for Enterococcus faecalis.
  • To compare the discriminatory power and concordance of MLVA with PFGE.

Main Methods:

  • Developed an MLVA typing scheme targeting seven genomic loci in Enterococcus faecalis.
  • Compared MLVA results with PFGE for 83 clinical isolates.
  • Assessed the concordance between the two typing methods.

Related Experiment Videos

Main Results:

  • The MLVA scheme identified 37 distinct genotypes among the 83 Enterococcus faecalis strains.
  • PFGE identified 31 genotypes.
  • MLVA and PFGE showed high concordance, with 90.4% agreement.

Conclusions:

  • MLVA is a robust and highly discriminatory molecular typing method for Enterococcus faecalis.
  • MLVA offers a valuable and efficient alternative to PFGE for epidemiological studies and outbreak investigations.
  • The developed MLVA scheme can aid in tracking the genetic diversity and transmission of Enterococcus faecalis.