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Interleukin-10 knockout mouse: a model for studying bone metabolism during intestinal inflammation
Stacey L Cohen1, Aideen M Moore, Wendy E Ward
1Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, Ontario, Canada, M5S 3E2.
Inflammatory Bowel Diseases
|October 9, 2004
Summary
Interleukin-10 knockout (IL-10 KO) mice exhibit bone abnormalities, including lower bone mineral content and density, alongside intestinal inflammation. This model is suitable for studying inflammation-associated bone loss and potential treatments.
Area of Science:
- Biomedical Science
- Immunology
- Orthopedics
Background:
- Interleukin-10 (IL-10) plays a crucial role in immune regulation.
- Dysregulation of IL-10 is implicated in various inflammatory conditions.
- Understanding IL-10's impact on bone health is vital for inflammatory disease research.
Purpose of the Study:
- To evaluate the suitability of the IL-10 knockout (KO) mouse model for studying inflammation-associated bone abnormalities.
- To compare bone mass and biomechanical strength between IL-10 KO and wild-type (WT) mice.
- To assess the influence of genotype and gender on bone parameters in the context of inflammation.
Main Methods:
- Comparison of bone mineral content (BMC) and bone mineral density (BMD) in femurs and lumbar vertebrae.
- Assessment of biomechanical strength parameters of femurs and lumbar vertebrae.
- Analysis of colonic histologic injury scores and serum proinflammatory cytokines (IL-6, IL-1beta, TNF-alpha).
- Study included male and female mice up to 13 weeks of age (n = 15-19/group).
Main Results:
- IL-10 KO mice displayed higher colonic injury scores and elevated serum proinflammatory cytokines compared to WT mice.
- Femur and vertebral BMC and BMD were significantly lower in IL-10 KO mice.
- Certain biomechanical strength parameters, including femur yield load and resilience, and lumbar vertebra peak load, were reduced in IL-10 KO mice.
- Males generally exhibited greater bone mass and strength than females, with a significant genotype x gender interaction for femur resilience.
Conclusions:
- IL-10 KO mice develop significant bone abnormalities concurrent with intestinal inflammation and elevated pro-inflammatory cytokines.
- The IL-10 KO mouse model is appropriate for investigating the mechanisms of inflammation-associated bone loss.
- This model holds potential for evaluating therapeutic interventions targeting bone abnormalities in inflammatory diseases.