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Related Experiment Videos

Cell transport via electromigration in polymer-based microfluidic devices.

Malgorzata A Witek1, Suying Wei, Bikas Vaidya

  • 1Department of Chemistry, Louisiana State University, Baton Rouge, LA 70803, USA.

Lab on a Chip
|October 9, 2004
PubMed
Summary
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This study demonstrates selective cell introduction into microfluidic devices by controlling electrokinetic transport of Escherichia coli and baker's yeast. Different buffer concentrations and polymer materials enable separation of E. coli and red blood cells.

Area of Science:

  • Biomedical Engineering
  • Microfluidics
  • Cellular Transport

Background:

  • Electrokinetic phenomena are crucial for manipulating cells in microfluidic systems.
  • Polymer microfluidic devices offer tunable surface properties for controlling cell behavior.
  • Understanding cell migration in microchannels is key for developing advanced separation techniques.

Purpose of the Study:

  • To evaluate electrokinetic transport of Escherichia coli and Saccharomyces cerevisiae (baker's yeast) in microfluidic devices.
  • To investigate the influence of polymer material (PMMA, PC) and surface modification (UV) on cell mobility.
  • To establish a method for selective cell introduction into microfluidic devices based on differential migration.

Main Methods:

  • Fabrication of microfluidic devices from pristine and UV-modified poly(methyl methacrylate) (PMMA) and polycarbonate (PC).

Related Experiment Videos

  • Measurement of apparent mobilities (micro(app)) of Escherichia coli and baker's yeast cells under varying buffer concentrations (PBS, 0.5 mM to 20 mM, pH 7.4).
  • Demonstration of selective cell introduction using Escherichia coli and red blood cells (RBCs) as a model system, monitored by laser backscatter.

    • Main Results:

    • Chip-to-chip reproducibility of cell mobilities was approximately 10% RSD.
    • Baker's yeast exhibited highest apparent mobility in UV-modified PC (0.5 mM PBS) and lowest in pristine PMMA (20 mM PBS).
    • E. coli cells migrated towards the anode in low ionic strength (0.5-1 mM PBS) and towards the cathode in high ionic strength (20 mM PBS), indicating decreased electrophoretic mobility.
    • Differential migration of E. coli and RBCs was observed, enabling selective introduction into microfluidic devices by adjusting buffer concentration and polymer type.

    Conclusions:

    • Electrokinetic transport of microbial and mammalian cells can be effectively controlled in polymer microfluidic devices.
    • Surface modification and buffer ionic strength significantly impact cell electrophoretic mobility and electroosmotic flow.
    • Differential cell migration provides a basis for selective cell introduction and separation in microfluidic applications.