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DNA Isolation

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DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

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Updated: Jul 1, 2026

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

Published on: August 6, 2008

Sequential DNA hybridisation assays by fast micromixing.

Martin Heule¹, Andreas Manz

¹Imperial College London, Dept. Chemistry, London, UKSW7 2AY. m.heule@hispeed.ch

|October 9, 2004

Summary

This study introduces a novel microfluidic device for rapid DNA hybridization assays. The system detects sequence differences, including single base-pair mismatches, in seconds using fluorescence detection.

Area of Science:

Biotechnology
Analytical Chemistry
Microfluidics

Background:

DNA hybridization assays are crucial for genetic analysis.
Traditional methods can be time-consuming and require large sample volumes.
Microfluidic devices offer potential for faster and more sensitive assays.

Purpose of the Study:

To explore DNA hybridization assays using a novel sequential scheme on a microfluidic device.
To investigate the kinetics of hybridization and detect sequence differences rapidly.
To overcome diffusion limitations in laminar flow for enhanced reaction efficiency.

Main Methods:

Utilized a 10x5 mm microfluidic device with a split channel for rapid mixing and a meandering channel for observation.
Employed DNA oligomers (20-mers) and fluorescence detection with DNA-intercalating dyes.

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Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

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DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems

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A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

Published on: November 14, 2018

Investigated two operational modes: continuous flow and stopped flow, analyzing fluorescence evolution over time.

Main Results:

Successfully detected 2 base-pair mismatches within 5-20 seconds under standard salt conditions.
Identified single base-pair mismatches under low salt conditions.
Reduced assay times to as low as 1-7 seconds in stopped-flow mode, while avoiding photobleaching in continuous flow.

Conclusions:

The developed microfluidic device enables rapid and sensitive DNA hybridization assays.
The system effectively detects sequence variations, including single base-pair mismatches.
This approach offers a significant advancement in assay speed and efficiency for genetic analysis.