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[A novel oligonucleotide arrays-based multiplex amplifiable probe hybridization technology].
He-Ping Liu1, Hong Wang, Zu-Hong Lu
1Key Laboratory of Molecular and Biomolecular Electronics, Ministry of Education, Department of Biomedical Engineering, Southeast University, Nanjing 210096, China. liuheping@seu.edu.cn
Yi Chuan Xue Bao = Acta Genetica Sinica
|October 12, 2004
Summary
Multiplex amplifiable probe hybridization (MAPH) combined with oligonucleotide arrays offers a novel, high-throughput method for measuring DNA copy number changes. This technology improves parallel assay capabilities for genetic disease detection.
Area of Science:
- Molecular Biology
- Genetics
- Biotechnology
Context:
- Multiplex amplifiable probe hybridization (MAPH) is an emerging technique for detecting gene copy number variations in genetic disorders.
- Traditional methods for analyzing MAPH products can be labor-intensive and limit high-throughput applications.
Purpose:
- To develop and validate a novel oligonucleotide array-based MAPH technology for precise DNA fragment copy number measurement.
- To enhance the parallel assay capability and efficiency of MAPH for genetic analysis.
Summary:
- A new MAPH method utilizes locus-specific amplifiable probes hybridized to genome DNA immobilized on nylon membranes.
- Collected probes are amplified using biotinylated primers and hybridized to oligonucleotide arrays containing DMD gene probes.
- Fluorescence imaging and quantitative analysis via ScanArray and ImageJ software enable relative quantification of DNA copy number changes.
Impact:
- This integrated approach replaces traditional agarose gel electrophoresis, significantly improving the throughput and scalability of genetic copy number analysis.
- Demonstrates the feasibility of high-throughput, relative quantification of DNA copy number variations in a single reaction, applicable to genetic diseases like DMD.