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Updated: Aug 21, 2026

Establishing 3D Endometrial Organoids from the Mouse Uterus
Published on: January 6, 2023
Early embryo-endometrial signaling modulates the regulation of matrix metalloproteinase-3
Shirly Lahav-Baratz1, Hanna Shiloh, Mara Koifman
1Department of Obstetrics and Gynecology, IVF Unit, Carmel Medical Center, Haifa, Israel. lahav@adi.org il
Objective:
To examine matrix metalloproteinase-3 (MMP-3) expression in human stromal cell culture after P stimulation and the effect of conditioned medium from human embryo-epithelial cells coculture on its expression and activity.
Design:
Metabolic and endocrine studies on human tissue.
Setting:
In vitro fertilization (i.v.f.) unit and endocrine research unit.
Patient(S):
Infertile patients undergoing endometrial tissue sampling for dating at the luteal phase before i.v.f.
Intervention(S):
Endometrial sampling and collection of human embryos culture media.
Main Outcome Measure(S):
Expression and activity of secreted MMP-3 by P-induced stromal cells, and in stromal cells exposed to conditioned medium from embryo-epithelial cell coculture.
Result(S):
Expression and activity of MMP-3 in human stromal cells decrease after P induction. Following incubation of these stromal-derived decidual cells with conditioned medium from embryo-epithelial cell coculture, MMP-3 expression and activity increased in a statistically significant manner.
Conclusion(S):
Progesterone inhibition of MMP-3 expression and its support of endometrial integrity were prevented by local expression of MMP-3 in response to embryonic signaling.
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