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Updated: Aug 21, 2026

Functional Assessment of Kinesin-7 CENP-E in Spermatocytes Using In Vivo Inhibition, Immunofluorescence and Flow Cytometry
Published on: December 28, 2021
Normal expression of isoforms activating cyclic adenosine monophosphate responsive element modulator in patients with
Irina Palermo1, Giuseppe Arcidiacono, Nunziata Barone
1Department of Biomedical Sciences and Unit of Pathology and Cytodiagnosis, University of Catania, Catania, Italy.
Objective:
To evaluate whether defective cyclic adenosine monophosphate responsive element modulator (CREM) expression is the causative factor of spermatid maturation arrest (SMA).
Design:
Comparative evaluation of the testicular histology in patients with SMA or normal spermatogenesis.
Setting:
University clinic of andrology.
Patient(S):
Azoospermic patients undergoing testicular biopsy.
Intervention(S):
None.
Main Outcome Measure(S):
Expression of CREMtau in quantitative immunohistochemistry analysis of testicular biopsy samples.
Result(S):
Regular CREM expression was observed in the tubules with round, but not elongated, spermatids of patients with SMA (n = 9). Quantitative analysis showed that round spermatids of patients with SMA had a staining intensity similar to that observed in controls (n = 7).
Conclusion(S):
Lack of spermatid elongation was not due to defective CREM expression. Therefore, CREM did not play a pathogenetic role in the onset of SMA in humans.
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