Related Experiment Video
Updated: Aug 21, 2026

Functional Cloning Using a Xenopus Oocyte Expression System
Published on: January 30, 2016
Molecular cloning, structure, expression, and chromosomal localization of the human Osterix (SP7) gene
Yuguang Gao1, Andrew Jheon, Houman Nourkeyhani
1Canadian Institutes for Health Research (CIHR) Group in Matrix Dynamics, Faculty of Dentistry, University of Toronto, Fitzgerald Building, Room 239, 150 College Street, Toronto, Ontario, Canada M5S 3E2.
Abstract:
We report the isolation of the human orthologue of the mouse Osterix (Osx/Sp7) gene, a C2H2 zinc finger transcription factor of the SP gene family and putative "master" regulator of bone cell differentiation. The human SP7 cDNA encodes a putative 431 amino acid protein that contains three consecutive C2H2 zinc finger repeats. The SP7 protein is highly conserved between mice and humans with an overall sequence identity of 95%. The expression of a SP7 mRNA transcript of approximately 3.2 kb is restricted to bone-derived cell lines in vitro but undetectable in any adult tissues including mandibular bone by Northern blot hybridization. The specific expression of SP7 mRNA in osteoblasts in vivo was further confirmed by in situ hybridization on human embryonic tissues. The highly restricted expression pattern and the divergence of the sequence outside of the zinc finger region distinguish SP7 as a unique member of the SP family. The SP7 gene consists of two exons, with exon 2 containing most of the protein coding sequence. The gene locus was mapped to chromosome 12q13.13 by fluorescent in situ hybridization (FISH). The identification and initial characterization of the SP7 gene will facilitate the study of the molecular regulation of osteoblast differentiation in humans.
Related Concept Videos
Chromatin Position Affects Gene Expression
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the timing and level of...
Cell Specific Gene Expression
Cell Specific Gene Expression
Heterochromatin
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...
Inheritance of Chromatin Structures
Methods of Nuclear Reprogramming

