Related Experiment Video

Updated: Aug 21, 2026

A Simple Bioassay for the Evaluation of Vascular Endothelial Growth Factors
09:04

A Simple Bioassay for the Evaluation of Vascular Endothelial Growth Factors

Published on: March 15, 2016

The vascular endothelial growth factor family and its receptors

Alexander A Parikh1, Lee M Ellis

  • 1Department of Surgery-Surgical Oncology, Temple University School of Medicine, Fourth Floor, Parkinson Pavilion, 3401 North Broad Street, Philadelphia, PA 19140, USA. parikhaa@tuhs.temple.edu

Hematology/Oncology Clinics of North America
|October 12, 2004
PubMed

Insights

This study details vascular endothelial growth factor (VEGF) biology and its receptors, crucial for understanding antiangiogenic therapies in cancer treatment.

Area of Science:

  • Molecular Biology
  • Oncology
  • Biochemistry

Background:

  • Vascular Endothelial Growth Factor (VEGF) is a key signaling protein.
  • VEGF and its receptors regulate angiogenesis, the formation of new blood vessels.
  • Dysregulated angiogenesis is implicated in cancer development and progression.

Purpose of the Study:

  • To describe the fundamental biology of the VEGF system.
  • To elucidate the mechanisms regulating VEGF and its receptor expression.
  • To highlight the importance of VEGF system knowledge for anti-cancer therapy optimization.

Main Methods:

  • Literature review of VEGF and receptor biology.
  • Analysis of gene and protein expression regulation.
  • Synthesis of current understanding of VEGF signaling pathways.

Main Results:

  • Detailed description of VEGF isoforms and receptor tyrosine kinases (VEGFRs).
  • Explanation of transcriptional, post-transcriptional, and post-translational regulation of VEGF.
  • Identification of key signaling cascades initiated by VEGF binding.

Conclusions:

  • A comprehensive understanding of VEGF biology is essential for effective cancer treatment.
  • Targeting the VEGF pathway is a critical strategy in antiangiogenic therapy.
  • Further research into VEGF regulation can lead to improved antineoplastic regimens.

More Related Videos

Modified In Vivo Matrix Gel Plug Assay for Angiogenesis Studies09:03

Modified In Vivo Matrix Gel Plug Assay for Angiogenesis Studies

Published on: June 30, 2023

Incorporating Pericytes into an Endothelial Cell Bead Sprouting Assay06:21

Incorporating Pericytes into an Endothelial Cell Bead Sprouting Assay

Published on: February 16, 2018

Related Concept Videos

Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...
Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
Mechanism of Angiogenesis01:10

Mechanism of Angiogenesis

Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...
Overview of the Vascular System01:20

Overview of the Vascular System

The vascular system comprises an extensive network of arteries, capillaries, and veins. The vascular system can be broadly divided into the blood and lymphatic systems. Typically, blood vessels can be categorized into three histological regions: tunica intima, tunica media, and tunica adventitia. The tunica intima consists of a single layer of endothelial cells attached to the basal lamina. Underlying the basal lamina is a connective tissue layer and an elastic lamina that gives stability and...
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR activation may...
Transducer Mechanism: Enzyme-Linked Receptors01:27

Transducer Mechanism: Enzyme-Linked Receptors

Enzyme-linked receptors are cell-surface receptors acting as an enzyme or associating with an enzyme intracellularly. They make excellent drug targets. Drugs can bind to the extracellular ligand-binding domain or directly affect their enzymatic domain and alter their activity.
Major types that are helpful drug targets include: