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Updated: Aug 21, 2026

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Characterization of an RNA bulge structure by Fourier transform infrared spectroscopy
Martina Banyay1, Jessica Sandbrink, Roger Strömberg
1Department of Biochemistry and Biophysics, Arrhenius Laboratories, Stockholm University, Stockholm SE-106 91, Sweden.
Abstract:
There may be several advantages associated with an antisense oligonucleotide that induces a bulged structure into its RNA target molecule. Many structures of RNA bulges are elucidated from single-stranded RNA models. However, a two-component system is the minimum requirement for a realistic antisense model. We have used Fourier transform infrared spectroscopy to investigate a single-stranded RNA oligonucleotide with known NMR solution structure, constructed to model a five nucleotide bulge, and its two-component oligonucleotide counterpart. The infrared spectra show A-helical base-paired stems and non-base-paired loops in both systems. The nucleosides are mainly in an anti-conformation. Both N-type and S-type of sugar puckers can be inferred from the infrared region sensitive to sugar conformations. The S-type of sugar pucker is likely to be associated with the nucleotides in the bulge. The FTIR results display an overall structural similarity between the two model systems.
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