Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Conditionally immortalized human colorectal crypt cell line].

Yong-liang Zhu1, Xian Zhong, Shu Zheng

  • 1The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, China.

Zhejiang Da Xue Xue Bao. Yi Xue Ban = Journal of Zhejiang University. Medical Sciences
|October 12, 2004
PubMed
Summary

Researchers established a novel human colorectal crypt cell line for in vitro cancer research. This immortalized cell line serves as a valuable model for studying colorectal carcinogenesis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Application of SPI-guided analgesia in laparoscopic gynecologic surgery: a randomized controlled trial evaluating the remifentanil-sparing effect and predictive value of time-weighted SPI.

Journal of clinical monitoring and computing·2026
Same author

Theoretical design of one-dimensional dual site metal-organic framework ribbons for the CO<sub>2</sub>RR.

Physical chemistry chemical physics : PCCP·2026
Same author

Two-Dimensional Metal-Organic Framework (TM<sub>3</sub>X<sub>12</sub>C<sub>24</sub>) as Promising Cathodes for Li-O<sub>2</sub> Batteries.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

STING-NF-κB signaling builds an influenza spillover barrier.

Science (New York, N.Y.)·2026
Same author

Functional characterization of CASP, a <i>CUX1</i> isoform, reveals its tumor-promoting role in colorectal cancer via TRIM21-mediated signaling.

iScience·2026
Same author

Novel bacterium <i>Enterocloster</i> sp. M3 promotes colorectal tumorigenesis via the production of the carcinogen styrene.

Gut microbes·2026

Area of Science:

  • Cell Biology
  • Gastroenterology
  • Cancer Research

Context:

  • Colorectal cancer (CRC) research requires reliable in vitro models.
  • Existing cell lines may not fully recapitulate native colorectal crypt cell behavior.
  • The development of immortalized human colorectal crypt cells is crucial for advancing CRC studies.

Purpose:

  • To establish a stable, immortalized human colorectal crypt cell line.
  • To characterize the key markers and properties of the newly developed cell line.
  • To validate its suitability for in vitro carcinogenesis research.

Summary:

  • Human fetal colorectal crypt cells were isolated and cultured.
  • Cells were immortalized using human telomerase reverse transcriptase (hTERT) and simian virus 40 large T antigen (SV40 LT).

Related Experiment Videos

  • Immunohistochemical and Western blot analyses confirmed the expression of specific markers (mucin, cytokeratin-pan, 8, 19, hTERT, SV40 LT) and Musashi-1 mRNA. The cells showed no signs of carcinogenesis in vivo or in vitro.

    • Impact:

    • The established cell line provides a unique in vitro model for studying colorectal cancer development.
    • This model facilitates research into the mechanisms of colorectal carcinogenesis.
    • It offers a platform for testing potential therapeutic interventions for colorectal cancer.