Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Specific binding of a basic peptide from HIV-1 Rev.

J Kjems1, B J Calnan, A D Frankel

  • 1Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139-4307.

The EMBO Journal
|March 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Improving wheat as a source of iron and zinc for global nutrition.

Nutrition bulletin·2019
Same author

Enhancing mineral bioavailability from cereals: Current strategies and future perspectives.

Nutrition bulletin·2018
Same author

Antibacterial isoeugenol coating on stainless steel and polyethylene surfaces prevents biofilm growth.

Journal of applied microbiology·2017
Same author

Circular RNAs in cancer: opportunities and challenges in the field.

Oncogene·2017
Same author

Leveraging Industry-Academia Collaborations in Adaptive Biomedical Innovation.

Clinical pharmacology and therapeutics·2016
Same author

Mucin-mediated nanocarrier disassembly for triggered uptake of oligonucleotides as a delivery strategy for the potential treatment of mucosal tumours.

Nanoscale·2015

The Rev protein

Area of Science:

  • Molecular Biology
  • Virology
  • Biochemistry

Background:

  • Human immunodeficiency virus type I (HIV-1) requires the Rev protein for cytoplasmic expression of viral mRNA.
  • Rev protein function depends on binding to the Rev-responsive element (RRE) in the viral env gene.

Purpose of the Study:

  • To investigate the specific binding interaction between the Rev protein and its target RNA sequence (IIB RNA).
  • To determine the minimal structural requirements for Rev-RRE interaction.

Main Methods:

  • RNA footprinting assays
  • Modification interference experiments
  • Mutational analysis of the RRE IIB RNA sequence

Main Results:

  • A 17-amino acid peptide from the Rev basic domain specifically binds to the 37-nucleotide IIB RNA.

Related Experiment Videos

  • Both the full Rev protein and the peptide interact with nucleotides at single-double stranded RNA junctions and within a helical region.
  • Rev binding likely induces secondary structure in both the RNA and protein.

    • Conclusions:

    • The RNA-binding domain of the Rev protein is small and sufficient for specific RRE recognition.
    • RNA-protein interactions can induce structural changes in both molecules, highlighting dynamic binding mechanisms.
    • This study provides insights into the molecular mechanisms of HIV-1 gene regulation.