An electrochemical biosensor for nitric oxide based on silver nanoparticles and hemoglobin

Area of Science:

• Electrochemistry
• Nanomaterials Science
• Biomedical Engineering

Background:

• Nitric oxide (NO) is a crucial signaling molecule.
• Developing sensitive and selective NO biosensors is essential for biological and medical research.
• Silver nanoparticles (AgNPs) offer unique electrochemical properties.

Purpose of the Study:

• To fabricate a stable and highly sensitive nitric oxide (NO) biosensor.
• To enhance the electrochemical reactivity and detection sensitivity of hemoglobin for NO sensing.
• To evaluate the performance of the developed NO biosensor.

Main Methods:

• Fabrication of a biosensor utilizing silver nanoparticles (AgNPs) and hemoglobin.
• Electrochemical characterization of the AgNP-hemoglobin interface.
• Determination of the linear concentration range, detection limit, and sensitivity for NO detection.
• Assessment of interference from co-existing compounds.

Main Results:

• The silver nanoparticle-based NO biosensor demonstrated high sensitivity, selectivity, and stability.
• AgNPs preserved hemoglobin microstructures while enhancing its electrochemical reactivity.
• The biosensor exhibited a linear concentration range from 1.0 x 10(-6) to 5.0 x 10(-5) M.
• A low detection limit of 3.0 x 10(-7) M and a sensitivity of 0.0424 microA microM(-1) NO were achieved.
• No interference was observed from potentially co-existing compounds.

Conclusions:

• The developed AgNP-hemoglobin biosensor is a promising tool for sensitive and selective nitric oxide detection.
• This approach offers enhanced electrochemical performance for biological sensing applications.
• The biosensor's stability and resistance to interference make it suitable for complex biological samples.