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Nuclear magnetic resonance spectroscopy to determine the micellar cholesterol in human bile
J P Ellul1, G M Murphy, H G Parkes
1Gastroenterology Unit, United Medical School of Guy's Hospital, London, UK.
FEBS Letters
|March 23, 1992
Abstract:
The cholesterol of gallstones comes from the vesicular rather than the micellar phase of bile. Progress in this field has been limited because conventional analytical methods disturb the distribution of cholesterol between the two phases. The resonance of the cholesterol C6 proton occurs at a chemical shift of 5.4 ppm, to be shown by 2D NMR to be specific for biliary cholesterol, and arises only from the micellar mode. Thus integration of the C6 proton resonance peak area provides a direct non-invasive determination of the cholesterol distribution in human bile.