The Canine Parvovirus wild-type strains infections in dogs--epidemiological and diagnostic aspects

Biological material was taken from dogs with diarrhea. Faecal samples were taken from live animals white intestinal tract fragments (i.e. small intestine, and stomach) were taken from dead animals. In total, 18 specimens were investigated from dogs housed alone or in large groups. The samples were examined for presence of viral infections and concurrent bacterial and parasitic infestations. To test for the presence of the viral infection, latex (On Site Biotech, Sweden) and direct immunofluorescence tests were performed. At the same time to the presence of CPV infection, was conducted by the PCR method with primers complementary to a conservative region of VP1/VP2. In order to identify the bacterial strain, the material was inoculated onto appropriate media and identified with API tests, whilst parasitological examinations were performed with Fulleborn's method. CPV infection was accompanied by CCV and CAV infections, as well as bacterial ones, caused mostly by Escherichia coli.