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Related Experiment Videos

A density-sensing factor controls development in Dictyostelium.

R Jain1, I S Yuen, C R Taphouse

  • 1Howard Hughes Medical Institute, Rice University, Houston, Texas 77251-1892.

Genes & Development
|March 1, 1992
PubMed
Summary
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Scientists identified a novel protein, conditioned medium factor (CMF), that regulates gene expression in Dictyostelium development based on cell density. This factor synchronizes cell aggregation, crucial for organism development.

Area of Science:

  • Cellular and Molecular Biology
  • Developmental Biology
  • Biochemistry

Background:

  • Cell density influences gene expression during Dictyostelium development.
  • This regulation is mediated by conditioned medium factor (CMF), an 80-kD glycoprotein.
  • CMF is slowly secreted and sensed by starved cells.

Purpose of the Study:

  • To isolate the cDNA encoding CMF.
  • To investigate the eukaryotic cell density-sensing mechanism.
  • To elucidate the role of CMF in Dictyostelium development.

Main Methods:

  • Isolation of a cDNA encoding CMF.
  • Amino acid sequence analysis of CMF.
  • Generation of CMF antisense transformants.
  • Restoration of development with purified CMF protein.

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Main Results:

  • The CMF amino acid sequence shows no similarity to known proteins, suggesting a new class of intercellular signal.
  • CMF antisense transformants failed to aggregate.
  • Addition of purified CMF restored normal development in transformants.

Conclusions:

  • CMF is a novel eukaryotic intercellular signal involved in cell density sensing.
  • CMF plays a critical role in synchronizing Dictyostelium development.
  • CMF triggers aggregation when cells reach a critical starvation density.