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Cloning and characterization of a gene encoding extracellular metalloprotease from Streptomyces lividans
H S Lichenstein1, L A Busse, G A Smith
1Amgen Inc., Thousand Oaks, CA 91320.
Gene
|February 1, 1992
Abstract:
The prt gene, encoding a protease (Prt) from Streptomyces lividans TK24, was cloned and sequenced. An S. lividans host with plasmid-borne prt secreted 200 micrograms/ml of a 22-kDa Prt into the culture medium. Prt is classified as a metalloprotease since its activity is significantly inhibited by 1,10-phenanthroline or EDTA. The region upstream from prt codes for an incomplete open reading frame (ORF) oriented opposite to prt. This ORF has a strong similarity to a gene family (lysR) whose members regulate the transcription of structural genes required for either biosynthesis or degradation.