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A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
Published on: April 11, 2014
An optimized method for the assay of the red blood cell--age-related enzyme aspartate aminotransferase
Yohan Robinson1, Edgar Cristancho, Dieter Böning
1Charité, Campus Benjamin Franklin, Institute of Sports Medicine, 12200 Berlin, Germany. yohan.robinson@charite.de
Laboratory Hematology : Official Publication of the International Society for Laboratory Hematology
|October 14, 2004
Abstract:
Three methods of preparation of red blood cell concentrate for erythrocyte aspartate aminotransferase measurement were compared: (1) filtration of whole blood through a cellulose column (n = 36); (2) washing of whole blood and aspiration of buffy coat after centrifugation (n = 48); (3) optimized method with washing without aspiration of buffy coat (n = 229).
