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Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine
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Modulation of human dendritic cell phenotype and function by probiotic bacteria.

A L Hart1, K Lammers, P Brigidi

  • 1Antigen Presentation Research Group, Faculty of Medicine Imperial College London, Northwick Park Campus, Watford Road, Harrow, Middlesex HA1 3UJ, UK.

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Probiotic bacteria, particularly bifidobacteria, modulate dendritic cell function and immune responses. This research clarifies how probiotics influence early immune cell interactions for potential inflammatory bowel disease treatments.

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Area of Science:

  • Immunology
  • Microbiology

Background:

  • Probiotics show promise for inflammatory bowel diseases, but their mechanisms and effective strains are unclear.
  • Dendritic cells are key in immune recognition and T cell response shaping, potentially mediating probiotic effects.

Purpose of the Study:

  • To investigate how different probiotic bacteria impact dendritic cell function.
  • To understand the role of dendritic cells in mediating probiotic effects on immune responses.

Main Methods:

  • Cultured human immune cells (mononuclear cells, whole blood, dendritic cells) with VSL#3 probiotic components.
  • Analyzed dendritic cell maturation markers and cytokine production using multicolour flow cytometry.
  • Assessed the impact on T cell polarization.

Main Results:

  • VSL#3 induced IL-10 and inhibited Th1 cell generation via dendritic cells.
  • Bifidobacteria strains showed significant anti-inflammatory effects, upregulating IL-10 and decreasing CD80.
  • VSL#3 counteracted LPS-induced inflammation by modulating IL-12 and IL-10 production.

Conclusions:

  • Probiotic bacteria exhibit varied immunomodulatory activities.
  • Probiotics influence immune response polarization at the dendritic cell antigen presentation stage.