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Related Experiment Videos

Bone allografting: an Indian experience.

Harish V Kurup1, Prabhakar Rao, Dilip K Patro

  • 1Department of Orthopaedics, Jawaharlal Institute of Post Graduate Medical Education and Research (JIPMER), Pondicherry, 605006, India. harishvk@yahoo.com

International Orthopaedics
|October 14, 2004
PubMed
Summary

Ethylene oxide sterilization offers a cost-effective method for preparing bone allografts, proving successful in treating nonunions and bone lesions. This approach is viable for developing countries lacking advanced freeze-drying technology.

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Area of Science:

  • Orthopedic Surgery
  • Biomaterials Science
  • Sterilization Technologies

Background:

  • Freeze-drying is optimal for allograft preparation but is prohibitively expensive for developing nations.
  • Ethylene oxide sterilization's impact on osteoinductive capacity remains a point of debate.
  • Establishing a bone bank is crucial for providing allograft materials.

Purpose of the Study:

  • To evaluate the efficacy and safety of ethylene oxide sterilization for cancellous bone allografts.
  • To assess the clinical outcomes of using ethylene oxide-sterilized allografts in treating bone nonunions and lesions.
  • To determine the cost-effectiveness of this sterilization method in resource-limited settings.

Main Methods:

  • Cancellous bone from 40 patients was collected, cleaned, chemically processed, and sterilized using ethylene oxide gas.

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  • Grafts were doubly packed and stored for subsequent implantation.
  • Clinical and radiological follow-up was conducted on patients receiving the allografts.

    • Main Results:

    • Successful union was achieved in 13 out of 14 nonunion sites within 12 months (average 44.8 weeks).
    • Graft incorporation was observed in 8 benign bone lesion cases by 29 weeks.
    • A single deep infection occurred, indicating a low infection rate.

    Conclusions:

    • Cancellous allograft sterilized with ethylene oxide is effective for treating bone nonunions and filling cavities in benign bone lesions.
    • Ethylene oxide sterilization is a reliable, safe, and cost-effective option for bone allograft preparation, particularly in resource-limited environments.
    • The low infection rate supports the efficacy of ethylene oxide for allograft sterilization.