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[Influence of intestinal dysbacteriosis on immune and hematopoietec function in mice]
Qing-hong Liang1, Lin Zhang, Shu-cheng Duan
1Department of Pediatrics, The Third Hospital of Hebei Medical University, Shijiazhuang 050051, China.
Objective:
To study the effects of intestinal microflora alteration on specific and nonspecific immune function and hematopoietic function of mice.
Methods:
Sixty BALB/C mice were divided at random into two groups, experimental group and control group, with 30 mice in each. The mice in the experimental group were given kanamycin 50 mg while those in the control group were given distilled water intragastrically everyday for consecutive 10 days. After the 10 day treatment all the mice were sacrificed, and the cecal contents were collected for quantitative analysis of the intestinal bacterial flora. Certain indexes of immune function, including phagocytosis rate of macrophages, number of T lymphocytes positively stained by esterase and serum interleukin 2 (IL-2) content, and the weight of the spleen, granulocyte-macrophage colony stimulating factor etc. as indexes of hematopoietic function were determined.
Results:
In the group, the quantity of Enterobacteriaceae, Enterococcus, Bifidobacterium and Lactobacillus were significantly lower than that in the control group (P < 0.01). The number of PFC (plaque forming cells), the phagocytosis rate of macrophage, the number of T lymphocytes with positive NANE staining, the level of IL-2 significantly decreased when compared with that in the control group (P < 0.01). The weight of the spleen in the experimental group decreased when compared with that in the control group (P < 0.01). Levels of IL-3, GM-CSF, the total number of WBC and the proportion of neutrophil remarkably decreased as compared to that in the control group (P < 0.01). Analysis of the correlations between normal microflora, immunologic and hematopoietic indexes showed that marked positive correlations between the quantity of Bifidobacteria and each immune index including the levels of IL-3 and GM-CSF. There was a positive correlation between IL-2 and IL-3, IL-2 and GM-CSF as well.
Conclusion:
The application of antibiotics may cause changes in the structure and quantity of intestinal microflora. The dysbacteriosis may decrease the immune function of organism. The dysbacteriosis may decrease the hemopoietic function. The dysbacteriosis, the decrease in immune and hematopoietic function may affect one another. The balance in microecosystem should be emphasized and antibiotics should be applied rationally to reduce the side effects such as dysbacteriosis.
Insights
Antibiotic use significantly alters gut microflora, leading to decreased immune and hematopoietic functions in mice. Maintaining gut microecosystem balance and rational antibiotic use are crucial to prevent adverse effects.
Area of Science:
- Microbiology
- Immunology
- Hematology
Context:
- Intestinal microflora plays a critical role in host health.
- Antibiotic administration can disrupt the delicate balance of gut microbiota.
Purpose:
- To investigate the impact of intestinal microflora alteration on immune and hematopoietic functions in mice.
- To assess the relationship between gut dysbiosis and host physiological responses.
Summary:
- Kanamycin treatment in mice led to a significant reduction in beneficial bacteria (Bifidobacterium, Lactobacillus) and an increase in harmful bacteria.
- This dysbiosis resulted in decreased immune responses, including reduced macrophage phagocytosis and lower interleukin-2 (IL-2) levels.
- Hematopoietic function was also impaired, evidenced by decreased spleen weight, white blood cell counts, and colony-stimulating factors (IL-3, GM-CSF).
Impact:
- Antibiotic-induced gut dysbiosis negatively affects both immune and hematopoietic systems.
- Restoring gut microecosystem balance is essential for maintaining host health.
- Rational antibiotic application is recommended to mitigate adverse effects on the host.
