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Updated: Aug 21, 2026

Use of Bisection to Reduce Mitochondrial DNA in the Bovine Oocyte
Published on: July 6, 2022
An optimized protocol of a human-to-cattle interspecies somatic cell nuclear transfer
Kyung H Chang1, Jeong M Lim, Sung K Kang
1School of Agricultural Biotechnology, Seoul National University, Seoul, Korea.
Abstract:
Human somatic cells were transferred into cattle enucleated oocytes, and a prospective, randomized study was designed to optimize donor cell preparation, fusion medium, and culture method. As a result, improved development of interspecies embryos was achieved by employing serum-starved cord fibroblasts, with Ca(2+)/Mg(2+)-free fusion medium and a serum-free medium being used for bovine embryos.
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