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Related Experiment Videos

Cold adaptation in budding yeast.

Babette Schade1, Gregor Jansen, Malcolm Whiteway

  • 1Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec, Canada H4P 2R2. babette.schade@mcgill.ca

Molecular Biology of the Cell
|October 16, 2004
PubMed
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Budding yeast Saccharomyces cerevisiae exhibits distinct early and late transcriptional responses to cold. The late response overlaps with environmental stress response (ESR) and is regulated by Msn2p/Msn4p, while the early response remains uncharacterized.

Area of Science:

  • Molecular Biology
  • Yeast Genetics
  • Stress Response

Background:

  • Cold shock triggers significant cellular changes in Saccharomyces cerevisiae.
  • Understanding yeast's transcriptional adaptation to cold is crucial for cellular resilience research.

Purpose of the Study:

  • To elucidate the transcriptional reprogramming of Saccharomyces cerevisiae upon cold exposure.
  • To differentiate early and late gene expression patterns during cold stress.

Main Methods:

  • Utilized DNA microarrays to quantify transcript abundance changes in yeast cells exposed to 10°C.
  • Analyzed gene expression data to identify distinct temporal response groups.

Main Results:

  • Identified two distinct transcriptional responses: early and late cold response.

Related Experiment Videos

  • Late cold response genes significantly overlap with environmental stress response (ESR) genes and involve Msn2p/Msn4p activators.
  • Early cold response genes are Msn2p/Msn4p-independent, suggesting a novel regulatory mechanism.

    • Conclusions:

    • Cold exposure induces a biphasic transcriptional response in yeast.
    • The late response mirrors general environmental stress responses, mediated by known activators.
    • A distinct, Msn2p/Msn4p-independent early response indicates a specific cold adaptation pathway.