Inter-simple sequence repeat markers and flow cytometry for the characterization of closely related Citrus limon
R Capparelli1, M Viscardi, M G Amoroso
1Zootechnical Sciences and Food Inspection Department, School of Agriculture, University of Naples, Federico II, Via Università 133, Portici, 80055 Naples, Italy. capparel@unina.it
Abstract:
The genetic relationship among commercial cultivars of Citrus limon (lemon) was analysed by inter-simple sequence repeats (ISSR) and flow cytometry techniques. Two cultivars with a close germplasm were distinguished by screening 10 SSR primers and by measuring DNA content of prestained nuclei.
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