Active clearance of human amyloid beta 1-42 peptide aggregates from the rat ventricular system

A major constituent of SP in the brains of Alzheimer's disease is 39-43 amino acid peptide called beta-amyloid peptide (Abeta). Recent data have demonstrated that Abeta has a strong tendency to form insoluble aggregates and that toxic effects of Abeta is based on its aggregation. In the current study, 100 microg of human synthetic Abeta 1-42 (sAbeta 1-42) was infused into the lateral ventricle of rat brain using a short-term infusion model. At 2 or 7 days following the infusion, sAbeta 1-42 was found to form insoluble aggregates, scattering throughout the entire ventricular systems. The sAbeta 1-42 aggregates were partially engulfed by phagocytic cells and deposited at the meningeal vessels or the choroid plexuses. However, these deposits mostly disappeared from the ventricles by 28 days post-infusion. Here, it is reported for the first time that considerable amounts of sAbeta 1-42 are almost cleared from the rat ventricular system by the mononuclear phagocytic system.