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Updated: Jun 18, 2026
A high-throughput method to globally study the organelle morphology in S. cerevisiae
Published on: March 2, 2009
[A new method for screening mutant yeast strains with green fluorescent protein]
Lian-mei Hu1, Ying-jie Piao, Cai-gu He
1Department of Histology and Embryology, Southern Medical University, Guangzhou 510515, China. hulianmei@yahoo.com.cn
Objective:
To establish a fast and simple method for screening mutant yeast strains.
Materials And Methods:
Homologous recombination technique was used to detect mutant yeast strains in yeast genomic library, with the green fluorescent protein gene as the reporter gene in the transposon.
Results:
The strains that emitted green fluorescence were isolated, indicating that the gfp gene was inserted into the yeast genome by homologous recombination.
Conclusion:
This study established a useful method for functional genome study by homologous recombination technique, and provide an alternative for gene therapeutic drug development.
