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Updated: Dec 31, 2025
In Vitro Three-Dimensional Sprouting Assay of Angiogenesis Using Mouse Embryonic Stem Cells for Vascular Disease Modeling and Drug Testing
Published on: May 11, 2021
[Construction of a three-dimensional human angiogenesis model in vitro for antiangiogenic drug selection]
1School of Pharmacy, Yunnan University, Kunming 650091, China. libin36@ynu.edu.cn
Objective:
To develop a simple human angiogenesis model that simulates all the critical steps of the angiogenic process for screening antiangiogenic substances.
Methods:
Human umbilical vein endothelial cells (HUVECs) were sandwiched into three-dimensional collagen gels for culturing to produce the angiogenesis model in vitro, on which the antiangiogenic effects of genistein, tyrphostin A23 and lavendustin C were assessed.
Results:
HUVECs had the capacity to repidly form highly organized capillary-like structures in this model with uniformity of the entire culture, and was easily reproducible. Tyrosine kinase inhibitors, genistein, tyrphostin A23 and lavendustin C were found to inhibit the capillary tube formation.
Conclusion:
This new human angiogenesis model has proved to be simple, responsive, and reproducible for testing potential angiogenic inhibitors, and is suitable for screening anti-angiogenic drugs.
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