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Fast and Accurate Exhaled Breath Ammonia Measurement
Published on: June 11, 2014
arrA is a reliable marker for As(V) respiration
D Malasarn1, C W Saltikov, K M Campbell
1Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA.
Science (New York, N.Y.)
|October 16, 2004
Summary
Arsenate-respiring bacteria impact arsenic contamination in water. The arrA gene is crucial for this process and can monitor bacterial activity in contaminated environments.
Area of Science:
- Environmental microbiology
- Geochemistry
- Molecular biology
Background:
- Arsenate-respiring bacteria influence arsenic speciation and mobility.
- Arsenic contamination of drinking water poses risks to human health.
Purpose of the Study:
- To investigate the role of the arrA gene in arsenate respiration.
- To determine if arrA can be used to detect and monitor As(V)-respiring bacteria.
Main Methods:
- Molecular genetics techniques were employed.
- The conservation and function of the arrA gene were studied.
- arrA expression was analyzed in contaminated sediments.
Main Results:
- The arrA gene is highly conserved among As(V)-respiring bacteria.
- arrA is essential for reducing arsenate to arsenite, especially when sorbed onto iron minerals.
- arrA presence and expression indicate active As(V)-respiring bacteria in iron-rich, arsenic-contaminated sediments.
Conclusions:
- The arrA gene is a reliable marker for identifying and monitoring As(V)-respiring bacteria.
- Monitoring arrA expression can help assess the role of these bacteria in controlling arsenic geochemistry at contaminated sites.
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