Related Experiment Video
Updated: Aug 21, 2026

Synthesizing Amino Acids Modified with Reactive Carbonyls in Silico to Assess Structural Effects Using Molecular Dynamics Simulations
Published on: April 26, 2024
Thiyl radical reaction with amino acid side chains: rate constants for hydrogen transfer and relevance for
Thomas Nauser1, Jill Pelling, Christian Schöneich
1Department of Pharmaceutical Chemistry, University of Kansas, 2095 Constant Avenue, Lawrence, Kansas 66047, USA.
Abstract:
Thiyl radicals are prominent intermediates during biological conditions of oxidative stress and have been suggested to be involved in the mutagenic effects of thiols. While several enzymatic processes rely on the formation and selective reactions of protein thiyl radicals with substrates, such reactions may represent a source for biological damage when occurring uncontrolled during oxidative stress. For example, intramolecular hydrogen transfer reactions to protein cysteine thiyl radicals may lead to secondary amino acid oxidation products, which may represent starting points for protein aggregation and/or fragmentation. Here, we have used a kinetic NMR method to determine rate constants, k(sc), for hydrogen transfer reactions between thiyl radicals and amino acid side chain C-H bonds at 37 degrees C. Rate constants cover a range between k(sc)
Related Concept Videos
Amino acids
Protein Folding
Protein Structure Is Critical to Its Biological Function
Proteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...
Protein Folding
tRNA Activation
Covalently Linked Protein Regulators
These groups modify specific amino acids in a protein.
What are Proteins?

