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[Optimization of the conditions for RAPD-PCR of Candida spp. and Cryptococcus spp]

M E Sansinforiano1, A Rabasco, M Martínez-Trancón

  • 1Departamento de Genética y Departamento de Patología Infecciosa, Facultad de Veterinaria, Universidad de Extremadura, Cáceres, España.

Revista Iberoamericana De Micologia
|October 19, 2004
PubMed

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...