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Updated: Aug 17, 2026

Isolation of Microvascular Endothelial Tubes from Mouse Resistance Arteries
Published on: November 26, 2013
Endothelium-derived hyperpolarizing factor (EDHF)
1Department of Physiology, Nagoya City University Medical School, Japan.
Abstract:
In vascular tissues, many types of stimulants which release endothelium-derived relaxing factor (EDRF) hyperpolarized the smooth muscle membrane in an endothelium-dependent manner. The hyperpolarizations were resistant to inhibitors of the actions of EDRF, thereby suggesting that they were elicited by an unidentified substance called endothelium-derived hyperpolarizing factor (EDHF). The EDHF induced hyperpolarization was produced by an increase in K(+)-permeability of the membrane, and was one of the factors to induce endothelium-dependent vasodilations. Release of EDHF required an increase in endothelial Ca2+, due either to release of Ca2+ from intracellular storage or to its influx from the external media.
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