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Biacore biosensor immunoassay for 4-nonylphenols: assay optimization and applicability for shellfish analysis
Jeanne V Samsonova1, Natalya A Uskova, Alexey N Andresyuk
1Department of Chemical Enzymology, Chemistry Faculty, M.V. Lomonosov Moscow State University, 119992 Moscow, Russia. jvs@enz.chem.msu.ru
Chemosphere
|October 19, 2004
Summary
A rapid biosensor immunoassay for 4-nonylphenols was developed. This method accurately detects 4-n-nonylphenol in shellfish, showing potential for ecological monitoring.
Area of Science:
- Environmental Chemistry
- Analytical Chemistry
- Biosensor Technology
Background:
- 4-Nonylphenols (4-NPs) are environmental contaminants.
- Accurate detection methods are crucial for ecological monitoring.
Purpose of the Study:
- To develop a rapid Biacore biosensor immunoassay for 4-nonylphenols.
- To evaluate the assay's applicability for shellfish analysis and ecological monitoring.
Main Methods:
- Development of a Biacore biosensor immunoassay using monoclonal and polyclonal antibodies.
- Immobilization of 9-(p-Hydroxyphenyl)nonanoic acid onto a sensor chip.
- Extraction and clean-up of 4-n-nonylphenol from shellfish samples using hexane and NH2 SPE columns.
Main Results:
- A limit of detection of 2 ng/mL for 4-n-nonylphenol was achieved with monoclonal antibodies.
- Shellfish analysis showed limits of detection around 10 ng/g.
- The assay successfully identified negative samples and detected 4-nonylphenols in two scallop samples.
Conclusions:
- The developed biosensor assay is a rapid and effective method for 4-nonylphenol detection in shellfish.
- This assay shows promise as a preliminary screening tool for ecological monitoring of 4-nonylphenols in marine environments.