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Updated: Aug 21, 2026
Echocardiographic Assessment of Cardiac Anatomy and Function in Adult Rats
Published on: December 13, 2019
Comparative analysis of the proteome of left ventricular heart of arteriosclerosis in rat
Zhen-Yu Huang1, Peng-Yuan Yang, Mohamad Radwan Almofti
1Department of Chemistry, Fudan University, 220 HanDan Road, Shanghai 200433, China.
Insights
Researchers identified altered proteins in rat hearts with coronary atherosclerotic heart disease (CAHD). This proteomic study reveals potential mechanisms and therapeutic targets for CAHD.
Area of Science:
- Cardiovascular Research
- Proteomics
- Animal Models
Background:
- Coronary atherosclerotic heart disease (CAHD) is a global health concern with poorly understood pathogenic mechanisms.
- Identifying molecular changes in CAHD is crucial for understanding disease progression and developing treatments.
Purpose of the Study:
- To investigate the proteomic alterations in the left ventricular myocardial tissue of a rat model of CAHD.
- To identify specific proteins that are differentially expressed in CAHD compared to control tissues.
Main Methods:
- Established a CAHD rat model using vitamin D3 and a high-fat diet for 6 weeks.
- Utilized two-dimensional electrophoresis (2-DE) to separate and analyze myocardial tissue proteomes.
- Employed micro high-performance liquid chromatography-mass spectrometry/mass spectrometry (micro-HPLC-MS/MS) and MALDI-TOF-MS for protein identification.
Main Results:
- Over 2500 polypeptides were separated, with 38 protein spots showing significant alterations in CAHD rats.
- 12 differentially expressed proteins were identified with high confidence.
- 26 abundant proteins were identified using micro-HPLC-MS/MS.
Conclusions:
- Proteomic analysis identified specific protein alterations associated with CAHD in a rat model.
- These findings contribute to understanding the pathogenetic mechanisms of CAHD.
- The identified protein alterations may offer prognostic and therapeutic insights for CAHD.
Abstract:
Despite the worldwide occurrence of coronary atherosclerotic heart disease (CAHD), the pathogenic mechanisms underlying this disease remain largely unknown. In this study, the experimental model of atherosclerosis in rat (CAHD rat) was established by the injection of vitamin D3 associated with high fat diet for 6 weeks. By using the proteomic approach, we comparatively analyzed the proteome of the control and CAHD rat left ventricular myocardial tissues. We reproducibly separated over 2500 polypeptides by using two-dimensional electrophoresis (2-DE) at pH range of 3-11. Among these proteins, 26 proteins with large amount were identified using micro high performance liquid chromatography mass spectrometer/mass spectrometer (micro-HPLC-MS/MS). Using PDQUEST software to process the 2-DE gel images, 38 protein spots that significantly altered in CAHD were detected. Of these, 12 proteins were identified with high confidence by using 2-DE and matrix-associated laser desorption ionization time-of-flight mass spectrometer (MALDI-TOF-MS). The identification of protein alterations specify to CAHD would clarify the pathogenetic mechanisms involved in the disease and might be of prognostic and therapeutic benefit.
