Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Effect of exercise on hepatic gene expression in rats: a microarray analysis.

Wataru Aoi1, Eiichiro Ichiishi, Naoyuki Sakamoto

  • 1Department of Internal Medicine, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan. waoi@koto.kpu-m.ac.jp

Life Sciences
|October 19, 2004
PubMed
Summary

Regular running exercise significantly alters liver gene expression, impacting nutrient metabolism and antioxidant capacity. Key signal transduction proteins like p38 and signal transducer and activator of transcription 3 (STAT3) are notably affected.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Progressive growth of a small gallbladder polyp diagnosed as intracholecystic papillary neoplasm: a case report.

BMC gastroenterology·2026
Same author

Clinicopathological features and immune checkpoint expression patterns in dMMR early gastric cancer: a retrospective pilot study.

BMC cancer·2026
Same author

The association between serum zinc level and clinical features in patients with inflammatory bowel disease.

Journal of clinical biochemistry and nutrition·2025
Same author

High neutrophil-to-lymphocyte ratio at <i>Helicobacter pylori</i> eradication increases the risk of eradication failure and post-eradication gastric cancer.

Scandinavian journal of gastroenterology·2024
Same author

[Real-World Data of Immunohistochemical Staining for DNA Mismatch Repair Proteins Highlight Candidates for Immune Checkpoint Inhibitor Treatment in Patients with Gastrointestinal Cancer].

Gan to kagaku ryoho. Cancer & chemotherapy·2024
Same author

An NLR paralog Pit2 generated from tandem duplication of Pit1 fine-tunes Pit1 localization and function.

Nature communications·2024

Area of Science:

  • Molecular Biology
  • Exercise Physiology
  • Hepatology

Background:

  • Exercise offers numerous benefits for liver function, including improved nutrient metabolism and enhanced antioxidant defenses.
  • The precise molecular pathways mediating these exercise-induced liver adaptations remain incompletely understood.

Purpose of the Study:

  • To investigate the molecular mechanisms of exercise-induced changes in liver function using gene expression analysis.
  • To identify specific genes and signaling pathways modulated by long-term running exercise in the rat liver.

Main Methods:

  • Utilized a high-density cDNA microarray with 2,845 gene probe sets to analyze liver gene transcription in rats.
  • Compared gene expression profiles between rats subjected to 4 weeks of running exercise and sedentary controls.

Related Experiment Videos

  • Validated key gene expression changes by measuring protein levels of p38 mitogen-activated protein kinase and signal transducer and activator of transcription 3 (STAT3).

    • Main Results:

    • Running exercise led to significant alterations in gene transcription, with 105 genes up-regulated and 86 genes down-regulated in the liver.
    • Identified changes in genes with known and unknown functions, highlighting the broad impact of exercise.
    • Observed increased protein levels of p38 and STAT3, correlating with increased mRNA levels detected by microarray analysis.

    Conclusions:

    • Long-term running exercise profoundly impacts liver function through significant modulation of gene expression.
    • Signal transduction pathways, particularly involving p38 and STAT3, are key molecular mediators of exercise-induced hepatic adaptations.
    • These findings provide molecular insights into how exercise benefits liver health.