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Human ORFeome version 1.1: a platform for reverse proteomics.

Jean-François Rual1, Tomoko Hirozane-Kishikawa, Tong Hao

  • 1Center for Cancer Systems Biology and Department of Cancer Biology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA.

Genome Research
|October 19, 2004
PubMed
Summary

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This summary is machine-generated.

Researchers created a human ORFeome (open reading frame proteome) collection with 8076 protein-encoding genes. This resource enables large-scale functional proteomics studies and future ORFeome improvements.

Area of Science:

  • Molecular Biology
  • Proteomics
  • Systems Biology

Background:

  • Systems biology requires comprehensive sets of protein-encoding open reading frames (ORFs) for functional proteome studies.
  • Existing resources needed improvement for large-scale cloning and functional analysis.

Purpose of the Study:

  • To generate a foundational human ORFeome collection (hORFeome v1.1) for systems biology research.
  • To establish a robust method for cloning nearly entire sets of human ORFs.

Main Methods:

  • Utilized an improved Gateway recombinational cloning approach.
  • Employed the Mammalian Gene Collection (MGC) resource as a starting point.
  • Cloned 8076 human ORFs as mini-pools of PCR-amplified products.

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Main Results:

  • Successfully generated the human ORFeome version 1.1 (hORFeome v1.1) collection.
  • The collection comprises 8076 human ORFs, representing at least 7263 human genes.
  • Demonstrated proteome-scale heterologous protein expression using hORFeome v1.1.

Conclusions:

  • hORFeome v1.1 is a valuable resource for large-scale human ORF cloning and functional proteomics.
  • This collection will facilitate diverse functional studies and serve as a basis for future ORFeome versions.