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Updated: Aug 21, 2026

A Fast Air-dry Dropping Chromosome Preparation Method Suitable for FISH in Plants
Published on: December 16, 2015
Chromosome identification and nomenclature of Sorghum bicolor
Jeong-Soon Kim1, Patricia E Klein, Robert R Klein
1Institute for Plant Genomics and Biotechnology, Texas A&M University, College Station, Texas 77843-2474, USA.
Abstract:
Linkage group identities and homologies were determined for metaphase chromosomes of Sorghum bicolor (2n = 20) by FISH of landed BACs. Relative lengths of chromosomes in FISH-karyotyped metaphase spreads of the elite inbred BTx623 were used to estimate the molecular size of each chromosome and to establish a size-based nomenclature for sorghum chromosomes (SBI-01-SBI-10) and linkage groups (LG-01 to LG-10). Lengths of arms were determined to orient linkage groups relative to a standard karyotypic layout (short arms at top). The size-based nomenclature for BTx623 represents a reasonable choice as the standard for a unified chromosome nomenclature for use by the sorghum research community.
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