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Huntingtin interacting protein 14 is an oncogenic human protein: palmitoyl acyltransferase
Charles E Ducker1, Erin M Stettler, Kevin J French
1Department of Pharmacology, H078, Penn State College of Medicine, 500 University Drive Hershey, PA 17033, USA.
Abstract:
Protein palmitoyltransferases (PATs) represent an exciting new target for anticancer drug design due to their pivotal roles in the subcellular localization of a number of oncogenes. We show that the Huntingtin interacting protein 14 (HIP14) is a PAT with a preference for the farnesyl-dependent palmitoylation motif found in H- and N-RAS. Characterization of HIP14 in mouse cells has revealed that it has the ability to induce colony formation in cell culture, anchorage-independent growth, and tumors in mice. Activity of the enzyme and its ability to transform cells is dependent on critical residues in the active site of the enzyme.
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