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Related Experiment Videos

Using Lactococcus lactis for glutathione overproduction.

Yin Li1, Jeroen Hugenholtz, Wilbert Sybesma

  • 1Wageningen Centre for Food Sciences, NIZO food research, P.O. Box 20, 6710 Ede, The Netherlands.

Applied Microbiology and Biotechnology
|October 19, 2004
PubMed
Summary
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Engineered Lactococcus lactis produced high levels of gamma-glutamylcysteine and glutathione. Supplementing cysteine increased glutathione production, demonstrating potential for fine-chemical production.

Area of Science:

  • Microbiology
  • Biotechnology
  • Biochemistry

Background:

  • Glutathione is a crucial tripeptide antioxidant in cellular defense.
  • Lactococcus lactis is a widely used lactic acid bacterium in food production.
  • Engineering microbial strains for enhanced metabolite production is a key area in biotechnology.

Purpose of the Study:

  • To engineer Lactococcus lactis for the production of gamma-glutamylcysteine and glutathione.
  • To investigate the effect of precursor amino acid supplementation on glutathione production.
  • To establish a high-level bacterial glutathione production system.

Main Methods:

  • Controlled expression of Escherichia coli genes gshA and gshB in Lactococcus lactis.
  • Cultivation of engineered strains in chemically defined medium.

Related Experiment Videos

  • Analysis of intracellular gamma-glutamylcysteine and glutathione concentrations.
  • Supplementation with cysteine to assess its impact on production.

    • Main Results:

    • High levels of gamma-glutamylcysteine were achieved with gshA or gshA and gshB expression.
    • Glutathione was successfully produced in strains expressing both gshA and gshB.
    • Cysteine supplementation significantly increased glutathione production and enzyme activity.
    • A record intracellular glutathione concentration of 358 nmol mg(-1) protein was attained.

    Conclusions:

    • Engineered Lactococcus lactis is a suitable host for efficient glutathione production.
    • The system demonstrates potential for fine-chemical production and as a model for food-related studies.
    • Cysteine's role in enhancing glutathione synthetase activity warrants further investigation.