Spectrophotometric determination of deacetylation degree of chitinous materials dissolved in phosphoric acid
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Updated: Aug 21, 2026

Measurement of Chitinase Activity in Biological Samples
Published on: August 22, 2019
Hsien-Yi Hsiao1, Chih-Cheng Tsai, Suming Chen
1Biosensor Laboratory, Department of Bio-Industrial Mechatronics Engineering, College of Bioresources and Agriculture, National Taiwan University, 136 Chou-Shan Road, Taipei, Taiwan 106, R.O.C.
Abstract:
A simple spectrophotometric method is proposed for determining deacetylation degrees (DD) of chitinous materials using phosphoric acid as the UV-transparent solvent system. Calibrating by the extinction coefficients (A(210)) of D-glucosamine and N-acetyl-D-glucosamine, DD values (24-88%) were computed numerically. The results correlated well (R(2) = 0.9805, n = 50) with those obtained by solid-state (13)C NMR. Comparison of the results obtained by the proposed UV method and solid-state (13)C NMR.
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