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Studies on the HPRT mutant frequency in T lymphocytes from healthy Indian male population as a function of age and
P R Vivek Kumar1, V Zareena Hamza, Mary N Mohankumar
1Radiological Safety Division, Indira Gandhi Centre for Atomic Research (IGCAR), Kalpakkam-603102, India.
Abstract:
Mutant frequency at the hypoxanthine-guanine phosphoribosyltransferase (HPRT) gene in the peripheral blood lymphocytes obtained from 44 healthy individuals (23 non-smokers and 21 smokers) of an Indian male population was studied using T-lymphocyte cloning assay. It was found that lnMF increased with age at a rate of 2.5% per year (P <0.001). Blood samples from smokers showed a significant (P <0.037) increase in HPRT mutant frequency (MF) (10.43 +/- 4.74 x 10(-6)) as compared to that obtained from non-smokers (7.69 +/- 3.69 x 10(-6)). This study also showed a significant (P <0.027) inverse correlation between lnMF and non-selected cloning efficiency (CE). However, with respect to age no variation was observed in cloning efficiency. The results obtained in this study showed a good comparison with those reported in different populations of the world.
