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Human osteoclast culture from peripheral blood monocytes: phenotypic characterization and quantitation of resorption

Katherine A Buckley1, Benjamin Y Y Chan, William D Fraser

  • 1Human Bone Cell Research Group, Department of Human Anatomy and Cell Biology, The University of Liverpool, UK.

Methods in Molecular Medicine
|October 20, 2004
PubMed
Abstract

No abstract available in PubMed .

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Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during bone...